Dr.RenalNash said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:
- No HPLC data: If there's no chromatography graph, it's not a real COA
- Exact 100% purity: Suspicious — real testing always shows slight variation
- No batch number: Can't trace back to specific production
- No endotoxin testing: For injectables, this is essential
- Template looks like it was made in Word: Professional labs have standardized formats
I have seen fake COAs that are literally photoshopped. Always verify independently if possible.
FranDenver said:One addition: an unstated method makes a report close to uninterpretable.
Genuinely useful, thank you. I had the facts and not the framework. Adding it to my notes with a link back to this thread.
Dr.RenalNash said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight. This confirms identity — not just purity.
Why this matters: HPLC can show you a "pure" compound, but if it's pure of the WRONG compound, purity is meaningless. Mass spec at m/z 4113.58 Da confirms it's actually semaglutide. My Janoshik report includes both HPLC and mass spec.
If your testing provider only offers HPLC, consider upgrading to include mass spec for at least your first batch from a new vendor.
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View Resultsanders_CPH said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
My vendor review relevant to certificates of analysis: ordered 5 times from my current compounder. Consistency has been excellent.
Shipping: Same-day local pickup
Packaging: Professional, discreet, proper labeling with lot/batch numbers
Purity: 98.6% average across orders (Janoshik verified)
Customer service: Responsive within 6 hours
Price: $142/month for tirzepatide 10mg equivalent