DataDave said:The provenance questions are more useful than the numbers.
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
anders_CPH said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
Third-party testing services for certificates of analysis besides Janoshik:
- Janoshik: Gold standard for peptide community, ~$144, 10-14 day turnaround
- Valitech: US-based, ISO 17025 accredited, ~$224
- Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
- ChemTox: Basic identity and purity testing, ~$114
Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.
DanielChem_CHI said:I am more sceptical of certificates as a category than this thread is.
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 15.3 minutes (consistent with semaglutide reference standard)
Peak purity: 98.1%
Related substances: 0.7% (single impurity at 9.2 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.6 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
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View ResultsOne thing that is still open after FranDenver’s answer:
Why two laboratories report different purity for the same batch, and which number to believe?
Closing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.