mike_nyc said:They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
I would rather be corrected than agreed with, if it comes to it.
maya_sedona said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
Third-party testing services for certificates of analysis besides Janoshik:
- Janoshik: Gold standard for peptide community, ~$157, 10-14 day turnaround
- Valitech: US-based, ISO 17025 accredited, ~$217
- Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
- ChemTox: Basic identity and purity testing, ~$127
Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.
LibrarianMeg said:I am more sceptical of certificates as a category than this thread is.
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 14.3 minutes (consistent with semaglutide reference standard)
Peak purity: 98.3%
Related substances: 0.4% (single impurity at 8.0 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.58 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
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View ResultsFollowing on from LipidDoc_ATL — and this may be the naive question:
Whether a purity figure and a content figure can disagree without one of them being wrong?
Reporting back.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.