Dr.KarenChen said:Inter-laboratory variation comes from real methodological differences, not sloppiness: which reference standard was used and how it was itself…
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.
Day 1: 100.7% purity
Day 14: 99.1% purity
Day 28: 99.5% purity
Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.
One thing that is still open after pat_auckland’s answer:
Why two laboratories report different purity for the same batch, and which number to believe?
kate.chem said:Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 16.7 minutes (consistent with semaglutide reference standard)
Peak purity: 98.8%
Related substances: 0.4% (single impurity at 10.2 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.5 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsClosing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.
BariatricNurseD said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Agreed, and mass spec answers a question purity does not: identity. High purity tells you one thing dominates the chromatogram, not that it is the molecule you ordered. A mass within about a dalton of expected is the identity check.